To investigate the relationship between a set of six candidate genes and the risk of diabetic retinopathy (DR) in a cohort of Chinese patients town community with type 2 diabetes mellitus (T2DM).
METHOD A population-based cross-sectional study. Diabetic subjects were recruited from the urban community in Beijing and categorized into groups of proliferative diabetic retinopathy (PDR), non-proliferative diabetic retinopathy (NPDR), or diabetes without retinopathy (DWR) by fundus photography and duration of diabetes.
Six candidate genes, including glycation end certain products receptor sophisticated (AGER), aldose reductase (AKR1B1), inducible nitric oxide synthase (iNOS), pigment epithelium derived factor (PEDF), tumor necrosis factor-alpha (TNF-α), and paraoxonase 1 (PON1), selected by Meta-analysis of genetic association study for DR and biochemical pathways involved in the development of DR. Allele and genotype distribution of 21 functional single nucleotide polymorphisms (SNPs) in their six candidate genes were investigated using the genotype MassARRAY system.
RESULTS Among the 1461 patients with diabetes were recruited from the community, 569 have been selected in the following genotypic analysis, including 97 patients with PDR, with NPDR 217, and 255 with DWR. For promoter variant rs1051993 in AGER gene, allele and genotype distribution in PDR group differ from those in the group DWR (allele: P = 0.011; genotype: P = 0.01).
Compared with DWR, patients with PDR had a lower frequency of heterozygous genotype GT (9.8% for DWR, 1% for PDR, OR: 0.10, 95% CI: 0.01 to 0.72) and minor alleles T (4.9% for DWR, 0.5% for PDR, OR: 0.10, 95% CI: 0.01 to 0.75). In the multivariate model, the distribution of genotypes of rs1051993 in PDR group were significantly different from those in the group DWR (GT vs GG: OR: 0.07, 95% CI: 0.01 to 0.61, P <0.001). No association with DR SNP observed in other genotypes.
CONCLUSION The data showed a significant association of rs1051993 variant in the gene promoter AGER with PDR in the Chinese group with type 2 diabetes.
Association of candidate gene polymorphisms with diabetic retinopathy in Chinese patients with type 2 diabetes.
Sector retinitis pigmentosa: Report of ten cases and review of the literature.
To describe the genotypic and phenotypic ten patients with retinitis pigmentosa sector (RP). We also review previously reported mutations associated with RP sector and provide discussions about possible underlying pathophysiological mechanisms.
Patients underwent detailed ophthalmological examination, fundus photography, fundus autofluorescence (FAF) imaging, spectral-domain optical coherence tomography (SD-OCT), as well as visual field and electroretinographic testing. All patients underwent a genetic test to identify the molecular etiology of their disease.
result A total of ten patients studied. Among these patients, nine had mutations in RHO (c.677T> C; p.Leu226Pro (Novel), c.68C> A; p.Pro23His, c.808A> C; p.Ser270Arg, c.44A> G; p .Asn15Ser, and c.325G> A; p.Gly109Arg), and one patient had a mutation in RPGR (c.3092_3093delAG; p.Glu1031Glyfs * 47). All patients with missense mutations in RHO have visual acuities (VAS) better than 20/30 and showed sustained ellipsoid zone retinal foveal and topped structure.
Patients with deletion c.3092_3093delAG in RPGR have a VA of 20/60 oculus dexter (OD) and the 20/400 oculus sinister (OS), as well as significant foveal contour thinning and atrophy. All patients showed changes in pigment, or marked atrophy along the lower arcade, or both. This pattern of degeneration associated with visual disabilities and hyperFAF and superior hypo.
Description: A polyclonal antibody for detection of p73 from Human, Mouse. This p73 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human p73 around the non-phosphorylation site of Y99
Description: A polyclonal antibody for detection of p73 from Human, Mouse. This p73 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human p73 around the non-phosphorylation site of Y99
Description: A polyclonal antibody for detection of p73 from Human, Mouse. This p73 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human p73 around the non-phosphorylation site of Y99
Description: A polyclonal antibody for detection of p73 from Human. This p73 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human p73 at AA range: 240-320
Description: A polyclonal antibody for detection of p73 from Human. This p73 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human p73 at AA range: 240-320
Description: A polyclonal antibody for detection of p73 from Human. This p73 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human p73 at AA range: 240-320
Description: A polyclonal antibody for detection of p73 from Human. This p73 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human p73 around the non-acetylation site of K321
Description: A polyclonal antibody for detection of p73 from Human. This p73 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human p73 around the non-acetylation site of K321
Description: A polyclonal antibody for detection of p73 from Human. This p73 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human p73 around the non-acetylation site of K321
Description: A polyclonal antibody for detection of p73 from Human, Mouse, Rat. This p73 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human p73 around the non-acetylation site of K327
Description: A polyclonal antibody for detection of p73 from Human, Mouse, Rat. This p73 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human p73 around the non-acetylation site of K327
Description: A polyclonal antibody for detection of p73 from Human, Mouse, Rat. This p73 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human p73 around the non-acetylation site of K327
Description: A polyclonal antibody for detection of p73 Acetyl Lys321) from Human. This p73 Acetyl Lys321) antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the human p73 around the acetylation site of K321
Description: A polyclonal antibody for detection of p73 Acetyl Lys321) from Human. This p73 Acetyl Lys321) antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the human p73 around the acetylation site of K321
Description: A polyclonal antibody for detection of p73 Acetyl Lys321) from Human. This p73 Acetyl Lys321) antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the human p73 around the acetylation site of K321
Description: A polyclonal antibody for detection of p73 Acetyl Lys327) from Human, Mouse, Rat. This p73 Acetyl Lys327) antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the human p73 around the acetylation site of K327
Description: A polyclonal antibody for detection of p73 Acetyl Lys327) from Human, Mouse, Rat. This p73 Acetyl Lys327) antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the human p73 around the acetylation site of K327
Description: A polyclonal antibody for detection of p73 Acetyl Lys327) from Human, Mouse, Rat. This p73 Acetyl Lys327) antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the human p73 around the acetylation site of K327
Description: A polyclonal antibody for detection of p73 phospho Tyr99) from Human, Mouse. This p73 phospho Tyr99) antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human p73 around the phosphorylation site of Y99
Description: A polyclonal antibody for detection of p73 phospho Tyr99) from Human, Mouse. This p73 phospho Tyr99) antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human p73 around the phosphorylation site of Y99
Description: A polyclonal antibody for detection of p73 phospho Tyr99) from Human, Mouse. This p73 phospho Tyr99) antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human p73 around the phosphorylation site of Y99
Description: A Rabbit Polyclonal antibody against p73 (phospho Tyr99) from Human/Mouse. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA
Description: A Rabbit Polyclonal antibody against p73 (phospho Tyr99) from Human/Mouse. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA
Description: A Rabbit Polyclonal antibody against p73 (Acetyl Lys327) from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, WB, ELISA
Description: A Rabbit Polyclonal antibody against p73 (Acetyl Lys327) from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, WB, ELISA
Description: A polyclonal antibody for p73 (pTyr99) from Human | Mouse. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human p73 around the phosphorylation site of tyrosine 99 (pTyr99).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This p73 (pTyr99) antibody is unconjugated.
Description: A polyclonal antibody for p73 (pTyr99) from Human | Mouse. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human p73 around the phosphorylation site of tyrosine 99 (pTyr99).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This p73 (pTyr99) antibody is conjugated to ATTO 390.
Description: A polyclonal antibody for p73 (pTyr99) from Human | Mouse. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human p73 around the phosphorylation site of tyrosine 99 (pTyr99).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This p73 (pTyr99) antibody is conjugated to ATTO 488.
Description: A polyclonal antibody for p73 (pTyr99) from Human | Mouse. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human p73 around the phosphorylation site of tyrosine 99 (pTyr99).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This p73 (pTyr99) antibody is conjugated to ATTO 565.
Description: A polyclonal antibody for p73 (pTyr99) from Human | Mouse. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human p73 around the phosphorylation site of tyrosine 99 (pTyr99).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This p73 (pTyr99) antibody is conjugated to ATTO 594.
Description: A polyclonal antibody for p73 (pTyr99) from Human | Mouse. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human p73 around the phosphorylation site of tyrosine 99 (pTyr99).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This p73 (pTyr99) antibody is conjugated to ATTO 633.
Description: A polyclonal antibody for p73 (pTyr99) from Human | Mouse. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human p73 around the phosphorylation site of tyrosine 99 (pTyr99).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This p73 (pTyr99) antibody is conjugated to ATTO 655.
Description: A polyclonal antibody for p73 (pTyr99) from Human | Mouse. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human p73 around the phosphorylation site of tyrosine 99 (pTyr99).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This p73 (pTyr99) antibody is conjugated to ATTO 680.
Description: A polyclonal antibody for p73 (pTyr99) from Human | Mouse. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human p73 around the phosphorylation site of tyrosine 99 (pTyr99).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This p73 (pTyr99) antibody is conjugated to ATTO 700.
Description: A polyclonal antibody for p73 (pTyr99) from Human | Mouse. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human p73 around the phosphorylation site of tyrosine 99 (pTyr99).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This p73 (pTyr99) antibody is conjugated to Alkaline Phosphatase.
Description: A polyclonal antibody for p73 (pTyr99) from Human | Mouse. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human p73 around the phosphorylation site of tyrosine 99 (pTyr99).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This p73 (pTyr99) antibody is conjugated to APC .
Description: A polyclonal antibody for p73 (pTyr99) from Human | Mouse. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human p73 around the phosphorylation site of tyrosine 99 (pTyr99).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This p73 (pTyr99) antibody is conjugated to APC/Cy7.
Description: A polyclonal antibody for p73 (pTyr99) from Human | Mouse. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human p73 around the phosphorylation site of tyrosine 99 (pTyr99).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This p73 (pTyr99) antibody is conjugated to Biotin.
Description: A polyclonal antibody for p73 (pTyr99) from Human | Mouse. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human p73 around the phosphorylation site of tyrosine 99 (pTyr99).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This p73 (pTyr99) antibody is conjugated to Dylight 350.
Description: A polyclonal antibody for p73 (pTyr99) from Human | Mouse. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human p73 around the phosphorylation site of tyrosine 99 (pTyr99).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This p73 (pTyr99) antibody is conjugated to Dylight 405.
Description: A polyclonal antibody for p73 (pTyr99) from Human | Mouse. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human p73 around the phosphorylation site of tyrosine 99 (pTyr99).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This p73 (pTyr99) antibody is conjugated to Dylight 488.
Description: A polyclonal antibody for p73 (pTyr99) from Human | Mouse. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human p73 around the phosphorylation site of tyrosine 99 (pTyr99).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This p73 (pTyr99) antibody is conjugated to Dylight 594.
Description: A polyclonal antibody for p73 (pTyr99) from Human | Mouse. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human p73 around the phosphorylation site of tyrosine 99 (pTyr99).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This p73 (pTyr99) antibody is conjugated to Dylight 633.
Description: A polyclonal antibody for p73 (pTyr99) from Human | Mouse. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human p73 around the phosphorylation site of tyrosine 99 (pTyr99).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This p73 (pTyr99) antibody is conjugated to FITC.
Description: A polyclonal antibody for p73 (pTyr99) from Human | Mouse. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human p73 around the phosphorylation site of tyrosine 99 (pTyr99).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This p73 (pTyr99) antibody is conjugated to HRP.
Description: A polyclonal antibody for p73 (pTyr99) from Human | Mouse. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human p73 around the phosphorylation site of tyrosine 99 (pTyr99).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This p73 (pTyr99) antibody is conjugated to PE/ATTO 594.
Description: A polyclonal antibody for p73 (pTyr99) from Human | Mouse. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human p73 around the phosphorylation site of tyrosine 99 (pTyr99).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This p73 (pTyr99) antibody is conjugated to PerCP.
Description: A polyclonal antibody for p73 (pTyr99) from Human | Mouse. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human p73 around the phosphorylation site of tyrosine 99 (pTyr99).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This p73 (pTyr99) antibody is conjugated to RPE .
Description: A polyclonal antibody for p73 (pTyr99) from Human | Mouse. The antibody is produced in rabbit after immunization with human synthetic phospho-peptide from human p73 around the phosphorylation site of tyrosine 99 (pTyr99).. The Antibody is tested and validated for WB, IHC, ELISA assays with the following recommended dilutions: WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000). This p73 (pTyr99) antibody is conjugated to Streptavidin.
Description: The p53 gene is a widely studied anti-oncogene, or tumor suppressor gene. The p53 gene product can act as a negative regulator of cell growth in response to DNA damage. Mutations and allelic loss of the p53 gene have been associated with malignant transformation in a wide variety of human tumors. p53 shares considerable sequence similarity with p73, a gene that maps to a region in chromosome 1 that is frequently deleted in neuroblastomas. However, p73 does not appear to be activated by DNA damaging agents.
Description: The p53 gene is a widely studied anti-oncogene, or tumor suppressor gene. The p53 gene product can act as a negative regulator of cell growth in response to DNA damage. Mutations and allelic loss of the p53 gene have been associated with malignant transformation in a wide variety of human tumors. p53 shares considerable sequence similarity with p73, a gene that maps to a region in chromosome 1 that is frequently deleted in neuroblastomas. However, p73 does not appear to be activated by DNA damaging agents.
Description: The p53 gene is a widely studied anti-oncogene, or tumor suppressor gene. The p53 gene product can act as a negative regulator of cell growth in response to DNA damage. Mutations and allelic loss of the p53 gene have been associated with malignant transformation in a wide variety of human tumors. p53 shares considerable sequence similarity with p73, a gene that maps to a region in chromosome 1 that is frequently deleted in neuroblastomas. However, p73 does not appear to be activated by DNA damaging agents.
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conclusion Sector RP is a rare form of RP in which only one or two quadrants of the retina display signs of clinical pathology. Most cases are caused by mutations in RHO. These data confirmed earlier reports phenotypic manifestations of RP sector. Low retinal quadrants may be more severely affected because of greater exposure to light.
Dave
